Localization and Nak regulation of endocytic machineries in Drosophila da dendrites
Date Issued
2009
Date
2009
Author(s)
Suo, Hsien
Abstract
The great complexity of dendrites is important for their proper functions on receiving and integrating signals. Although the cellular mechanisms regulating dendrite development are largely unknown, secretory and endocytic pathways were recently shown to be involved. Numb-associated kinase (Nak) is an endocytic kinase required for dendrite morphogenesis. Nak depleted da neurons showed decreased numbers of dendritic branches. YFP-Nak is highly co-localized with clathrin light chain (Clc)-GFP at the tips of growing dendrites; however, the molecular mechanisms of Nak in dendrites are still unclear. I am interested in dissecting the endocytic steps that Nak participates in. In this study, I demonstrated the differential distribution of Rab4-mRFP, Rab5-GFP, Rab11-GFP, and ManII-GFP along the dendritic processes, in which Rab4-mRFP and Rab11-GFP preferentially distributed in proximal or distal dendrites, respectively. This data suggest the distinct requirements of cellular activities in different regions of dendrites. Although YFP-Nak colocalized largely with all the examined endocytic markers, knocked-down Nak by RNAi did not exert significant effects on the numbers and distributions of these markers. Moreover, YFP-Nak showed different dynamics with Rab5-GFP and Rab4-mRFP. Collectively, Nak is not functioning in the establishment and maintenance of the polarized distributions of Rab4-mRFP, Rab5-GFP, Rab11-GFP, and ManII-GFP.
Subjects
endocytosis
dendrite
drosophila
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