行政院國家科學委員會專題研究計畫成果報告:鉤端螺旋體造成腎小管間質腎炎之機制及台灣人畜鉤端螺旋體症之流行病學(3/3)
Date Issued
2002
Date
2002
Author(s)
潘銘正
DOI
902313B002295
Abstract
The purpose of the plan is to
investigate the epidemiology of leptospirosis
in pigs, and military dogs and studies the
mechanism of tubulointerstitial nephritis by
pathogenic leptospira. A total of 659 pigs
and 127 military dogs were examined by
using standard microscopic agglutination test
(MAT). The pathogenic Leptospira shermani
outer membrane extract and recombinant
LipL32 protein were administered to cultured
mouse tubule cells. We use the RNA
differential display technology and subtracted
hybridization technology to search the
possible pathogenic factors. The
seroprevalence of leptospira in Taiwan were
57.7% in pigs and 77.2% in military dogs.
We found that outer membrane extract and
recombinant LipL32 protein could cause the
increase of iNOS, mRNA of MCP-1 and
TNF-a of mouse tubule cells. The
prospective study of prs gene from the RNA
differential display technology and genes
from subtracted hybridization technology can
help to understand the relationship of the
genes and pathogenic mechanism. The results
of the plan were high prevalence in animal in
Taiwan and the inflammation factors
expression increase of tubule cells causing by
outer membrane proteins.
investigate the epidemiology of leptospirosis
in pigs, and military dogs and studies the
mechanism of tubulointerstitial nephritis by
pathogenic leptospira. A total of 659 pigs
and 127 military dogs were examined by
using standard microscopic agglutination test
(MAT). The pathogenic Leptospira shermani
outer membrane extract and recombinant
LipL32 protein were administered to cultured
mouse tubule cells. We use the RNA
differential display technology and subtracted
hybridization technology to search the
possible pathogenic factors. The
seroprevalence of leptospira in Taiwan were
57.7% in pigs and 77.2% in military dogs.
We found that outer membrane extract and
recombinant LipL32 protein could cause the
increase of iNOS, mRNA of MCP-1 and
TNF-a of mouse tubule cells. The
prospective study of prs gene from the RNA
differential display technology and genes
from subtracted hybridization technology can
help to understand the relationship of the
genes and pathogenic mechanism. The results
of the plan were high prevalence in animal in
Taiwan and the inflammation factors
expression increase of tubule cells causing by
outer membrane proteins.
Subjects
leptospira
epidemiology
outer
membrane protein
membrane protein
RNA
differential display
differential display
subtracted
hybridization
hybridization
Publisher
臺北市:國立臺灣大學獸醫學系暨研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
902313B002295.pdf
Size
143.91 KB
Format
Adobe PDF
Checksum
(MD5):6369a68e3e35887196f98c8a808528bf