Development and measurement of two-photon-base fluorescence correlation spectroscopy
Journal
2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007
Pages
955-960
ISBN
9.78142E+12
Date Issued
2007
Author(s)
Abstract
We presented the fluorescence correlation spectroscopy (FCS) based on two-photon microscopy system to access physical parameters that give rise to fluctuations in fluorescence signal. As given excitation volume, the diffusion coefficient of molecules due to Brownian Movement into or out of excitation volume is determined. Further, depended on autocorrelation analysis based on diffusion coefficients of molecules, the fraction of bound species from measured samples containing the dye labeled anti-rabbit IgG and rabbit IgG can be separated. The mean standard deviation of diffusion coefficient measurement in our two-photon based system is about 0.064. The changes of diffusion coefficient with ISnM concentration solution of fluorescein are 246.173um∧2/s to 35.1626um∧2/s with different concentration solution of glycerol ranging from 2.5% to 40%. Moreover, the maximal binding fraction of kinetics mode and end mode between Anti-Rabbit IgG and Rabbit IgG is 60% and 73%, respectively. © 2007 IEEE.
Type
conference paper