Pleomorphic extra-renal manifestation of the glomerular podocyte marker podocalyxin in tissues of normal beagle dogs
Journal
Histochemistry and Cell Biology
Journal Volume
127
Journal Issue
4
Pages
399-414
Date Issued
2007
Author(s)
Abstract
Podocalyxin (PC) was initially identified as a major sialoprotein on the apical surface of glomerular podocytes to perform the filtration barrier function. Later, it was reported to be expressed in endothelial cells, megakaryotes/platelets, and hemangioblasts, the common progenitor cells of the hematopoietic and endothelial cells. Recently, increasing numbers of reports have indicated that PC is not merely a molecule restricted at renal glomerulus, angiogenic or hematopoietic system. To further elucidate the expression pattern and address the possible physiological role of PC in adult mammals, we conducted an extensive study by immunohistochemistry and immunofluorescence staining on various tissues of healthy adult beagle dogs. By combinatory usage of two different anti-podocalyxin antibodies recognizing distinct epitopes in PC, we have demonstrated that (1) PC is expressed in renal tubules, mesothelium, myocardium, striated muscles in tongue, esophagus and extraocular region, myoepithelial cells in esophagus and salivary glands, neurons, and ependyma, etc.; (2) there are at least three forms of PC proteins, depending upon the accessibility of two different PC antibodies, expressed in different organs/systems; and (3) a particular form of PC is distributed in a vesicle-like compartment in certain organs/systems, such as the central nervous system. ? 2006 Springer-Verlag.
SDGs
Other Subjects
epitope; podocalyxin; protein antibody; animal cell; animal tissue; antigen recognition; article; central nervous system; controlled study; dog; ependyma; esophagus; extraocular muscle; glomerulus; heart muscle; immunofluorescence test; immunohistochemistry; kidney tubule; mesothelium; myoepithelium cell; nerve cell; nonhuman; nucleotide sequence; podocyte; priority journal; protein expression; protein function; protein localization; salivary gland; skeletal muscle; tongue; Animals; Biological Markers; Blotting, Western; Bone Marrow Cells; Cell Line; Cell Line, Tumor; Digestive System; Dogs; Endocrine System; Eye; Female; Genitalia, Female; Genitalia, Male; Humans; Immune System; Immunohistochemistry; Kidney Glomerulus; Male; Myocardium; Nervous System; Podocytes; Sialoglycoproteins; Urinary Tract; Canis familiaris; Mammalia
Type
journal article