Transmission Mode of Watermelon silver mottle virus (WSMoV) by Thrips palmi Karny (Thysanoptera: Thripidae) and Effects of WSMoV on Life History of Thrips
Date Issued
2012
Date
2012
Author(s)
Chen, Wei-Te
Abstract
The melon thrips (Thrips palmi) is a pest on cucurbit crops but also a vector that transmits tospoviruses, for example Watermelon silver mottle virus (WSMoV). In this study, the transmission mode of WSMoV by T. palmi was clarified. The
results of transmission assay indicate that first -instar larvae, second-instar larvae and adults of T. palmi acquired the virus and transmitted the virus. However, adults had the highest transmission efficiency when they acquired the virus at first -instar stage. Using reverse transcription-polymerase chain reaction, the WSMoV M RNA’s complementary RNA of which ecodes virus nostructural protein was detected in virus-infected thrips. These results suggest that T. palmi transmits WSMoV in a persistent-propagative manner. Further, the effect of WSMoV infection on survival rate and developmental time of T. pami larvae was examined. The developmental time and survival rate of WSMoV-infected T. palmi larvae were not different from those of healthy thrips. When T. palmi fed on the WSMoV-infected plants, either healthy thrips or virus -infected thrips grew faster
than thrips fed on healthy plants. These results imply that WSMoV did not directly affect T. palmi, but WSMoV infection affects the developmental time indirectly through the WSMoV-infected plant. In feeding preference test of T. palmi adults, both male and female thrips prefered thrips-damaged plant and more males stayed on WSMoV-infected watermelon seedling, but females did not. Theese results indicate T. palmi is attracted by thrips damage and WSMoV infection. In this study, I not only exanined the transmission mode of WSMoV by Thrips palmi, but also studied on the interaction between virus and thrips vector. However, the effect of WSMoV infection on T. palmi should be studied in details to clarify the actual relationships between them.
Subjects
Tospovirus
persistent-propagative
survival rate
developmental time
feeding preference
Type
thesis
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