應用條件化基因剔除及轉基因策略探討小鼠C型血小板生長因子(PDGF-C)生理作用之分子調控機制(1/3)
Date Issued
2003
Date
2003
Author(s)
鄭登貴
DOI
912313B002384
Abstract
Several inducible gene expression systems have been developed in vitro in
recent years to overcome limitations with traditional transgenic mice. One of these,
the tetracycline-regulated system, has been used successfully in vivo. The expression
of the targeted protein are controlled by a promoter containing seven DNA operator
sequences from the Escherichia coli transposon 10 ( Tn10). To control the expression
of PDGF-C in transgenic (Tg) mice, we used a tetracycline-controlled transactivator
(tTA or rtTA) and a tTA/rtTA-dependent promoter linked to a PDGF-C gene.
Doxycycline (Dox)-sensitive co-regulation of two transcriptionally coupled
transgenes will be investigated in the mouse. For this, we generated four independent
mouse line clone carrying coding regions for mouse PDGF-C gene and green
fluorescent protein (GFP) in a bicistronic, bidirectional module under the control of
the Tet-responsive promoter and/or 4311 regulatory regions of trophoblast- specific
promoter. It is anticipated that the rtTA andor tTA-sensitive bi-directional expression
module is well suited to express genes of interest in a regulated manner and that GFP
can be used to track transcriptional activity of the module in the living mouse.
Subjects
PDGF-C
Tetracycline-regulated system
green fluorescent protein
transgenic mice
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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