Development of Thermosensitive Poly(N-Isopropylacrylamide-Co-((2- Dimethylamino) Ethyl Methacrylate))-Based Nanoparticles for Controlled Drug Release
Resource
NANOTECHNOLOGY v.22 n.26 pp.ARTN265608
Journal
NANOTECHNOLOGY
Journal Volume
v.22
Journal Issue
n.26
Pages
ARTN26560-8
Date Issued
2011
Date
2011
Author(s)
PENG, CHENG-LIANG
TSAI, HAN-MIN
YANG, SHU- JYUAN
LUO, TSAI-YUEH
LIN, WUU-JYH
SHIEH, MING- JIUM
SHIEH, MING-JIUM
Abstract
Thermosensitive nanoparticles based on poly(N- isopropylacrylamide-co-((2 -dimethylamino)ethylmethacrylate)) (poly(NIPA-co-DMAEMA)) copolymers were successfully fabricated by free radical polymerization. The lower critical solution temperature (LCST) of the synthesized nanoparticles was 41 C and a temperature above which would cause the nanoparticles to undergo a volume phase transition from 140 to 100 nm, which could result in the expulsion of encapsulated drugs. Therefore, we used the poly (NIPA-co- DMAEMA) nanoparticles as a carrier for the controlled release of a hydrophobic anticancer agent, 7- ethyl-10-hydroxy-camptothecin (SN-38). The encapsulation efficiency and loading content of SN-38-loaded nanoparticles at an SN-38/poly(NIPA-co-DMAEMA) ratio of 1/10 (D/P = 1/10) were about 80% and 6.293%, respectively. Moreover, the release profile of SN-38-loaded nanoparticles revealed that the release rate at 42 C (above LCST) was higher than that at 37 C (below LCST), which demonstrated that the release of SN-38 could be controlled by increasing the temperature. The cytotoxicity of the SN-38-loaded poly(NIPA-co-DMAEMA) nanoparticles was investigated in human colon cancer cells ( HT-29) to compare with the treatment of an anticancer drug, Irinotecan® (CPT-11) . The antitumor efficacy evaluated in a C26 murine colon tumor model showed that the SN-38- loaded nanoparticles in combination with hyperthermia therapy efficiently suppressed tumor growth. The results indicate that these thermo-responsive nanoparticles are potential carriers for controlled drug delivery.
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