Antimutagenic and antioxidative activities of intracellular extracts of bifidobacteria against reactive oxygen species
Date Issued
2004
Date
2004
Author(s)
Sheu, Ya-Jiun
DOI
zh-TW
Abstract
本研究探討六株雙叉桿菌 Bifidobacterium adolescentis BCRC14606、B. bifidum BCRC14615、B. breve BCRC11846、B. infantis BCRC14602、B. lactis Bb-12 與 B. longum BCRC14634 對活性氧物質的抗致突變性。使用安氏試驗法之測試菌株 Salmonella typhimurium TA102 來分析雙叉桿菌胞內物對氧化突變劑 tert-butylhydroperoxide (t-BOOH) 與過氧化氫 (H2O2) 誘導突變之防禦效果。結果顯示六株雙叉桿菌菌體懸浮液 (1010 CFU/mL) 經超音波破碎，離心 (15,000 × g, 30 min) 移除細胞碎片後所得到的胞內物，對 t-BOOH 和 H2O2 之抗致突變性分別為 15.9-50.9 % 和 51.5-72.3 %。更進一步研究結果顯示抗致突變性的主要機制為去致突變作用。而雙叉桿菌的抗氧化變異性可能歸因於其抗氧化能力，所以進一步評估其抗氧化性，檢測系統包括還原力、螫合亞鐵離子能力、DPPH 自由基及活性氧物質之清除能力探討。結果顯示六株雙叉桿菌之胞內物都表現出良好的還原力，以 B. lactis 表現最好，其還原力相當於 328 μg/mL 的抗壞血酸。清除 DPPH 自由基測定中，則以 B. longum 清除能力最好。螫合亞鐵離子方面，6 株雙叉桿菌胞內物表現出 34-52 % 之螫合力，以B. longum最佳。在清除活性氧物質方面，胞內物均具有清除超氧陰離子之能力，清除率介於 52.7-69.9 %，而以 B. adolescentis 效果最好；對過氧化氫之清除率則在 5.0-18.0 % 之間，其中以 B. bifidum有較佳表現；而在清除羥基自由基方面，B. bifidum 具有 11.8 % 之清除力，為 6 株雙叉桿菌中表現最佳者。
Antimutagenic activities of intracellular extracts of Bifidobacterium adolescentis BCRC14606, B. bifidum BCRC14615, B. breve BCRC11846, B. infantis BCRC14602, B. lactis Bb-12 and B. longum BCRC14634 against reactive oxygen substances were studied by a modified Ames test using Salmonella typhimurium TA102 as a test strain. The intracellular extracts were prepared by disrupting cells (1010 CFU/mL) with an ultrasonic disrupter and subsequently removing cell debris by centrifugation at 15,000 × g for 30 min. Assays for the ability of intracellular extracts of bifidobacteria against mutations induced by peroxide mutagens tert-butylhydroperoxide (t-BOOH, 10μg/plate) and hydrogen peroxide (H2O2, 6μmol/plate) toward S. typhimurium TA102 were developed. Results showed that the antimutagenic activities of bifidobacteria against t-BOOH and H2O2 were 15.9-50.9% and 51.5-72.3%, respectively. It was further shown that main mechanism of antimutagenicity is desmutagenic effect. The antimutagenic activities of bifidobacteria were probably attributed to their antioxidative activities. Therefore, antioxidative activities of these bifidobacteria were further determined by measuring their reducing activity, ferrous ion chelating activity
Subjects
雙叉桿菌
抗致突變性
抗氧化性
活性氧物質
antioxidative activity
bifidobacteria
antimutagenicity
reactive oxygen species
Type
thesis
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