Fluorometric biosensing of the total amino acid content and the glutamate content of green tea infusions using an automated multi-channel flow system
Journal
Bioscience, Biotechnology and Biochemistry
Journal Volume
60
Journal Issue
1
Pages
99-102
Date Issued
1996
Author(s)
Abstract
An automated multi-channel continuous-flow analyzing system was constructed for the rapid measurement of both the total amino acid content and the L-glutamate content of green tea infusions. L-Glutamate in samples was oxidized and deaminated enzymatically in a channel with a mini-reactor that was packed with L-glutamate oxidase immobilized on glass beads, and the evolved ammonium ions were measured by a fluorogenic reaction with o-phthalaldehyde and β-mercaptoethanol. The total amino acid content was measured in a channel with another mini-reactor which was filled with immobilized L-amino acid oxidase. The fluorometric method is not subject to interference by reducing substances such as tea catechins and ascorbate, and the analytes in tea infusions were measured without pretreatment of samples. The use of this system for evaluation of tea quality was investigated.se activity by NGF inducers and detected prostaglandins D2and E2as metabolites of arachidonic acid. From the observation that the induction of NGF by each NGF inducers was inhibited by cyclooxygenase inhibitors or dexamethasone, cyclooxygenase activation is supposed to be an essential process for NGF induction. © 1996, Taylor & Francis Group, LLC. All rights reserved.
SDGs
Type
journal article