2"-O-Methyl 反意核醣核酸應用於肺癌之基因治療(2/3)

Lung cancer is the cause of 12.8% of malignancy and leads to 17.8% of
cancer deaths worldwide. Lung cancer is also the leading cause of cancer
death in Taiwan. Annually, there were 6000 patients died of lung cancer in
Taiwan, emphasizing the need for new and effective treatment. Studies of the
inhibition results by exogenously added inhibitors have produced
contradictory results, ranging from immediate cell death, to decreased cell
proliferation after a long delay, to no change in proliferation. The potential of
telomerase inhibition as a therapeutic modality for human cancer remains
unknown. In the first year of this proposed project, we used 2'-O-methyl RNA for cell growth arrest of lung cancer through telomerase inhibition. 2'-O-methyl
RNA with complementary human telomerase RNA sequence was transfected
into a cultured lung cancer cells, CL1-5, using cationic liposome as a vector.
The cell growth was inhibited by the 2'-O-methyl RNA in comparison with the
control groups. The 2'-O-methyl RNA transfected cells showed decreased
telomerase activity and increased proportion of apoptotic cells. In the second
year, we applied the same methodology to another lung cancer cell lines,
A549. The cell growth of A549 cells was inhibited by the 2'-O-methyl RNA in
comparison with the control groups. The 2'-O-methyl RNA transfected cells
showed little decreased telomerase activity and increased proportion of
apoptotic cells. Next year, we will study the downstream gene expression
profile and invasion ability change of the 2'-O-methyl RNA transfected cells.