研發文心蘭功能性基因體以提昇其花卉品質與產量─文心蘭假球莖之養分儲存與開花品質及產期相關功能性基因體分析(2/2)
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
葉開溫
DOI
922317B002006
Abstract
The investigation of nutrient storage, floral quality and floral time in Oncidium Gower
Ramsey pseudobulb during the development of flower stalk were carried out. At the
initiation of flower stalk (length 2-4 cM), more than 1000 pseudobulb specific expressed
genes were discovered using cDNA microarray and subtractive EST library; The Northern
blot analysis of fourteen cDNA in the subtractive EST library related to carbohydrate
metabolism group confirm that thirteen cDNA have higher expression level in pseudobulb,
suggesting mucilage may be formed first and then transferred to starch during the
development of flower stalk. We also found that class III peroxidase (POXs) were highly
expressed in the subtractive EST library and obtained eleven POXs genes form Oncidium
cDNA library. Now these POXs genes have been transformed to Arabidopsis and
Oncidium plants to observe their effects on inflorescence and flower development.
Subjects
vOncidium
subtractive library
cDNA microarray
Publisher
臺北市：國立臺灣大學植物科學研究所
Type
journal article
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