Susceptibility of Vibrio parahaemolyticus to various environmentalstresses after cold shock treatment

Vibrio parahaemolyticus was subjected to cold shock treatment at 20 or 15 jC for 2 or 4 h. The effect of cold shock on the
survival of V. parahaemolyticus subjected to subsequent low temperature (5 and 18 jC) and other adverse conditions (47 jC,
6 ppm crystal violet, 1000 ppm H2O2, 25 mM acetic acid and 25 mM lactic acid) was investigated.
Regardless of the cold shock treatment, survival of V. parahaemolyticus increased when stored at 5 or 18 jC, while no
increase in survival was noted for cells cold shocked in the presence of chloramphenicol. Cold shock treatment under the
conditions tested, in general, enabled V. parahaemolyticus cells to survive better following subsequent challenge by crystal
violet, while the cold-shocked organism was more susceptible to high temperature (47 jC), H2O2 and organic acids (lactic and
acetic acid) than the non-shocked cells. Furthermore, the temperature and time of the cold shock treatment affected the cold
shock response of V. parahaemolyticus.