Urinary total arsenic and 8-hydroxydeoxyguanosine are associated with renal cell carcinoma in an area without obvious arsenic exposure
Journal
Toxicology and Applied Pharmacology
Journal Volume
262
Journal Issue
3
Pages
349-354
Date Issued
2012
Author(s)
Abstract
8-Hydroxydeoxyguanosine (8-OHdG) is one of the most reliable and abundant markers of DNA damage. The study was designed to explore the relationship between urinary 8-OHdG and renal cell carcinoma (RCC) and to investigate whether individuals with a high level of 8-OHdG would have a modified odds ratio (OR) of arsenic-related RCC. This case-control study was conducted with 132 RCC patients and 245 age- and sex-matched controls from a hospital-based pool between November 2006 and May 2009. Pathological verification of RCC was completed by image-guided biopsy or surgical resection of renal tumors. Urinary 8-OHdG levels were determined using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Concentrations of urinary arsenic species, including inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), were determined by a high performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. Level of urinary 8-OHdG was significantly associated with the OR of RCC in a dose-response relationship after multivariate adjustment. Urinary 8-OHdG was significantly related to urinary total arsenic. The greatest OR (3.50) was seen in the individuals with high urinary 8-OHdG and high urinary total arsenic. A trend test indicated that the OR of RCC was increased with one of these factors and was further increased with both (p=0.002). In conclusion, higher urinary 8-OHdG was a strong predictor of the RCC. High levels of 8-OHdG combined with urinary total arsenic might be indicative of arsenic-induced RCC. ? 2012 Elsevier Inc..
SDGs
Other Subjects
8 hydroxydeoxyguanosine; arsenic; arsenic acid; arsenic trioxide; cacodylic acid; methanearsonic acid; adult; arsenic poisoning; article; atomic absorption spectrometry; cancer patient; cancer risk; cancer surgery; controlled study; female; high performance liquid chromatography; hospital based case control study; human; kidney biopsy; kidney carcinoma; kidney tumor; liquid chromatography; major clinical study; male; multivariate analysis; risk; tandem mass spectrometry; tumor biopsy; urine level; Age Factors; Arsenic; Carcinoma, Renal Cell; Case-Control Studies; Chromatography, Liquid; Creatinine; Deoxyguanosine; Female; Humans; Kidney Neoplasms; Male; Middle Aged; Odds Ratio; Sex Factors; Taiwan; Tandem Mass Spectrometry
Type
journal article