L aser-induced fluorescence technique for DNA and proteins separated by capillary electrophoresis
Resource
Journal of Chromatography B (793), 37–48
Journal
Journal of Chromatography B
Pages
-
Date Issued
2003
Date
2003
Author(s)
DOI
246246/2006111501275593
Abstract
Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength.500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE–LIF using He–Ne lasers. These systems are benefited from low background, low costs, easy maintenance, & compactness. Derivatizations of DNA & proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, & high efficiency, the applications of CE–LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, & single cell analysis have been presented.
Subjects
Reviews
DNA
Proteins
Publisher
Taipei:National Taiwan University Dept Chem Engn
Type
journal article
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