The Effects of Dietary β-glucan on Non-specific Immune Response and Survival of Small Abalone (Haliotis diversicolor supertexta)
Date Issued
2006
Date
2006
Author(s)
Shie, Jeng-Tzyo
DOI
zh-TW
Abstract
This purpose of this study is aimed to know the effect of dietary β-glucan on non-specfic immune response in small abalone (Haliotis diversicolor supertexta) and use sodium alginate with β-glucan to observe survival and attachment of small abalone post-larvae.
In experiment 1 to compare agar (1%)+Spirulina powder (24.5%), agar (1%)+blue-green algae powder (24.5%), agar (1%)+Chlorella powder (24.5%), agar (1%)+Gracilaria powder (24.5%), agar (1%)+Ulva powder (24.5%),agar (1%)+Sodium alginate (24.5%),agar (1%),Sodium alginate (24.5%) to induce ingestion agents. The best treatment is Sodium alginate (24.5%).
In experiment 2 to compare the control (without β-glucan), 2, 4, 8, 16 g/kg β-glucan and blank (Gracilaria sp.) by observing the THC (total haemocyte counts), O2-, PR (phagocytic rate), PI (phagocytic index), PO (phenoloxidase), GOT (glutamate oxalacetate transaminase), GPT (glutamate pyruvate transaminase), luminol- and lucigenin- enhanced chemiluminescence. The result showed that dietary β-glucan can enhance THC, O2-, PR, PI, luminol- and lucigenin- enhanced chemiluminescence. In all treatments showed that no significant differences in SOD, GOT and GPT. That means β-glucan can enhance non-specfic immune responses in small abalone. The treatment in 4g/kg β-glucan is the best effect on O2- activity(0.563±0.004) and PR(0.199±0.014%).
In experiment 3 to compare sodium alginate and 4g/kg β-glucan by adding into agar (1%) to observe attachment number and survival. The result showed that sodium alginate can increase post-larvae of small abalone attachment number(219.66±9.07). This treatment of 4g/kg β-glucan add into agar (1%) can enhance post-larvae of small abalone survival on plate during 15 days.
Subjects
葡聚多醣
非特異性免疫反應
活存
藻酸鈉
β-glucan
Non-specific Immune Response
Survival
Abalone
Haliotis diversicolor supertexta
sodium alginate
Type
other
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