Properties of an adrenal medullary protein immuno-related to steroid acute regulatory (StAR) protein
Journal
Endocrine Research
Journal Volume
26
Journal Issue
4
Pages
737-745
Date Issued
2000
Author(s)
Abstract
Immunohistochemistry using a StAR peptide antiserum had previously revealed strong staining in rat and bovine adrenal medulla, suggesting the presence of a protein immunogenically related to StAR. Western blots of bovine medulla tissue homogenates showed the principal adrenal medullary immuno-reactive species to have a higher molecular weight (50 kDa) compared to StAR protein (30 kDa). Subcellular fractionation localised the 50 kDa species principally to the medulla cytosol. StAR peptide antiserum binding to both the 30 kDa and 50 kDa species could be specifically competed by the peptide antigen. These data suggest that the adrenal medullary immuno-reactive species and StAR protein are distinct entities, which share some features in common.
Other Subjects
steroidogenic acute regulatory protein; adrenal medulla; animal tissue; cattle; cell fractionation; conference paper; immunohistochemistry; molecular weight; nonhuman; priority journal; protein analysis; tissue homogenate; Western blotting; zona fasciculata
Type
conference paper