A novel human stem cell coculture system that maintains the survival and function of culture islet-like cell clusters
Journal
Cell Transplantation
Journal Volume
17
Journal Issue
6
Pages
657-664
Date Issued
2008
Author(s)
Abstract
Islet-like cell clusters (ICCs) have been suggested to be a source of insulin-producing tissue for xenotransplantation in type 1 diabetes. We designed an approach to maintain the cultured rat pancreatic ICC survival and function, when cocultured with human umbilical cord mesenchymal stem cells (HUMSCs). HUMSCs in coculture have the ability to maintain ICC survival and function, for which number and insulin secretion of ICCs are increasing and lasting for 3 months, while ICCs gradually crash, which results in cell death after a period of 12 days of culture without HUMSCs. Cytokine protein array showed it has more than a twofold increase in levels of several cytokines (interleukin-6, tissue inhibitor of metalloproteinases-1, tissue inhibitor of metalloproteinases-2, monocyte chemoattractant protein-1, growth related oncogene, hepatocyte growth factor, insulin-like growth factor binding proteins 4, and interleukin-8) on coculture medium, implying an important role of these cytokines in this coculture system. These findings suggest that coculture with HUMSCs may have a significant potential to protect ICCs from damage during culture, and may be employed in a novel culture approach to maintain islet cell survival and function before transplantation. Copyright ? 2008 Cognizant Comm. Corp. All rights reserved.
Subjects
Coculture; Cytokines; Human umbilical cord mesenchymal stem cells; Islet-like cell clusters
SDGs
Other Subjects
insulin; interleukin 6; interleukin 8; monocyte chemotactic protein 1; scatter factor; somatomedin binding protein 4; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2; cytokine; animal cell; article; cell culture; cell death; cell function; cell isolation; cell protection; cell regeneration; cell survival; cell type; coculture; cord blood stem cell transplantation; culture medium; cytokine production; human; human cell; insulin release; islet like cell cluster; nonhuman; oncogene; pancreas islet; priority journal; protein microarray; rat; stem cell; animal; culture technique; cytology; evaluation; mesenchymal stem cell; metabolism; methodology; microarray analysis; physiology; Sprague Dawley rat; Rattus; Animals; Cells, Cultured; Coculture Techniques; Culture Techniques; Cytokines; Humans; Islets of Langerhans; Mesenchymal Stem Cells; Microarray Analysis; Rats; Rats, Sprague-Dawley
Type
journal article