miR-122 targets an anti-apoptotic gene, Bcl-w, in human hepatocellular carcinoma cell lines
Resource
Biochemical and Biophysical Research Communications 375 (3): 315-320
Journal
Biochemical and Biophysical Research Communications
Pages
315-320
Date Issued
2008
Date
2008
Author(s)
Lin, Cliff Ji-Fan
Gong, Hong-Yi
Tseng, Hung-Chia
Wang, Wei-Lun
Wu, Jen-Leih
Abstract
miR-122, a hepato-specific microRNA (miRNA), is frequently down-regulated in human hepatocellular carcinoma (HCC). In an effort to identify novel miR-122 targets, we performed an in silico analysis and detected a putative binding site in the 3′-untranslated region (3′-UTR) of Bcl-w, an anti-apoptotic Bcl-2 family member. In the HCC-derived cell lines, Hep3B and HepG2, we confirmed that miR-122 modulates Bcl-w expression by directly targeting binding site within the 3′-UTR. The cellular mRNA and protein levels of Bcl-w were repressed by elevated levels of miR-122, which subsequently led to reduction of cell viability and activation of caspase-3. Thus, Bcl-w is a direct target of miR-122 that functions as an endogenous apoptosis regulator in these HCC-derived cell lines. ? 2008 Elsevier Inc. All rights reserved.
Subjects
Bcl-w; Hepatocellular carcinoma; miR-122
SDGs
Other Subjects
caspase 3; microRNA; protein bcl w; 3' untranslated region; apoptosis; article; Bcl w gene; binding site; cancer cell culture; cell viability; enzyme activation; gene; gene targeting; human; human cell; liver cell carcinoma; mir 122 gene; nucleotide sequence; priority journal; protein blood level; 3' Untranslated Regions; Apoptosis; Apoptosis Regulatory Proteins; Base Sequence; Carcinoma, Hepatocellular; Caspase 3; Cell Survival; Down-Regulation; Enzyme Activation; Genetic Vectors; Humans; Liver Neoplasms; MicroRNAs; Molecular Sequence Data; RNA, Small Interfering; Transfection
File(s)![Thumbnail Image]()
Loading...
Name
16.pdf
Size
787.69 KB
Format
Adobe PDF
Checksum
(MD5):de1953d7f08f0c3b7d4531309f67fdc2