Presence of the extended-spectrum-β-lactamase and plasmid-mediated ampc-encoding genes in escherichia coli from companion animals—a study from a university-based veterinary hospital in Taipei, Taiwan
Journal
Antibiotics
Journal Volume
10
Journal Issue
12
Date Issued
2021
Author(s)
Abstract
Extended-spectrum-β-lactamase (ESBL) and AmpC β-lactamase are two enzymes com-monly found in Enterobacteriaceae that confer resistance to major antibiotics, such as third-generation cephalosporins that are widely prescribed for both human and animals. We screened for Escherichia coli producing ESBL and plasmid-mediated AmpC β-lactamase (pAmpC) from dogs and cats brought to National Taiwan University Veterinary Hospital, Taipei, Taiwan from 29 June 2020, to 31 December 2020. The genotypes and phylogenetic relatedness of these E. coli were also analyzed. Fifty samples of E. coli obtained from 249 bacterial isolates were included in this study. Among them, eight isolates had ESBL, seven had pAmpC, and one had both. Thirty-two percent (16/50) of E. coli isolates were resistant to third-generation cephalosporins. The detected ESBL genes included the blaCTX-M-1 and blaCTX-M-9 groups, and the blaCMY-2 group was the only gene type found in pAmpC. ESBL-producing E. coli belonged to the pathogenic phylogroup B2, and the sequence types (STs) were ST131 and ST1193. Three isolates were determined to be ST131-O25b, a highly virulent epidemic clone. The pAmpC-producing E. coli were distributed in multiple phylogroups, primarily the commensal phylogroup B1. The STs of the pAmpC-producing E. coli included ST155, ST315, ST617, ST457, ST767, ST372, and ST93; all of these have been reported in humans and animals. Imipenem was active against all the ESBL/pAmpC-producing E. coli; however, since in humans it is a last-resort antimicrobial, its use in companion animals should be restricted. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Companion animals
Escherichia coli
Extended-spectrum-β-lactamase
Plasmid-mediated AmpC
amoxicillin plus clavulanic acid
ampicillin
beta lactamase AmpC
cefotaxime
ceftazidime
ceftiofur
cephalosporin
clavulanic acid
enrofloxacin
extended spectrum beta lactamase
imipenem
animal experiment
antibiotic resistance
antibiotic sensitivity
Article
ascites fluid analysis
bacterial gene
bacterium isolate
body mass
conjugation
controlled study
gene sequence
genotype
genotyping technique
minimum inhibitory concentration
multidrug resistance
multidrug resistant bacterium
multilocus sequence typing
nonhuman
pet animal
phenotype
phylogeny
polymerase chain reaction
rhinorrhea
Taiwan
urinalysis
zone of inhibition
Type
journal article