Fabrication and release behavior of a novel freeze-gelled chitosan/γ-PGA scaffold as a carrier for rhBMP-2
Resource
Dental Materials 22 (7): 622-629
Journal
Dental Materials
Journal Volume
22
Journal Issue
7
Pages
622-629
Date Issued
2006
Date
2006
Author(s)
Abstract
Objective: The aim of this study was to fabricate a novel composite porous scaffold by blending chitosan and gamma-poly(glutamic acid) (γ-PGA) for the sustained delivery of rhBMP-2. Methods: Chitosan and γ-PGA were blended to fabricate a novel porous scaffold by the freeze-gelation method. For comparison, scaffolds made of freeze-dried chitosan, freeze-dried PLLA, and freeze-gelled chitosan were also prepared. The scaffolds were loaded with rhBMP-2, and then the controlled release of rhBMP-2 from the scaffolds was assessed by ELISA. Results: The freeze-gelled chitosan/γ-PGA scaffold (M0=318.29 ng, k=0.32 d-1) gave the most satisfactory release curve, followed by the freeze-gelled chitosan (M0=392.76 ng, k=0.59 d-1), freeze-dried chitosan (M0=229.21 ng, k=2.28 d-1), and freeze-dried PLLA (M0=8.4 ng, k=482.54 d-1) scaffolds. In the stability test, p-dioxane (the solvent for PLLA) seriously deteriorated rhBMP-2, whereas acetic acid (the solvent for chitosan) did not. Significance: A novel chitosan/γ-PGA composite scaffold for the controlled release of rhBMP-2 was established, with an enhanced release amount and sustained release behavior. This scaffold has many potential applications in bone regenerative therapies. © 2005 Academy of Dental Materials.
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journal article
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