Molecular Epidemiology of G3 Rotaviruses in Northern Taiwan
Date Issued
2008
Date
2008
Author(s)
Lin, Hsueh-Ching
Abstract
Rotavirus is the most important pathogen causing infantile diarrhea worldwide. Prophylaxis of rotavirus infection is important due to unavailability of efficient treatment. Monitoring genetic variation and evolution of rotavirus is essential for estimating the efficiency of developing vaccines and providing information for vaccine research.otaviruses are formed by three layers of proteins. The outer layer is composed of outer capsid proteins VP7 and VP4, and the middle layer is formed by VP6 protein. The inner core contains 11 segments of double stranded RNA genome.otaviruses are divided into groups A~G depending on the antigenicity of VP6. Most human rotaviruses belong to group A and are classified into subgroups I and II. Based on VP7 and VP4 genes, group A rotaviruses are further differentiated into 15 G types and 27 P types; according to NSP4 gene, group A rotavirus can be classified into genotypes A~E. Besides, according to the position of the tenth and eleventh gene segments on RNA polyacrylamide gel electrophoresis, rotavirus RNA can be differentiated into long or short eletropherotype.lobally, epidemiological studies of rotavirus revealed that the most common types include G1P[8], G3P[8], G4P[8], and emerging G9P[8], characterized by subgroup II and long electropherotype, and G2P[4] characterized by subgroup I and short electropherotype.his study investigated the genetic characteristics of G3 rotavirus genome in northern Taiwan from 1983 to 2005. G3 rotaviruses in northern Taiwan appeared in two major periods, early period (1983~1991) and late period (1997~2005).he VP7, VP4, NSP4, and VP6 genes of these Taiwanese G3 rotaviruses from the two periods were amplified by RT-PCR, and then sequenced, and phylogenetically analyzed. In the phylogenetic analysis of the VP7 genes, G3 rotaviruses can be classified into G3-I (1983~1991) and G3-II (1997~2005). For the VP4 genes, G3 rotaviruses can be differentiated into P[8]-I (1983~1990) and P[8]-II (1990~1991 and 1997~2005). For the NSP4 genes of Taiwanese strains, three lineages, B-I (1985~1991), B-II (1997 and 2001), and B-III (1999 ~2005) were found. For the VP6 genes, II-1 (1985~1991, 2002 ~2005) and II-2 (1997~2005) were identified. Both nucleotide and amino acid identities within the same lineages in each phylogenetic analysis were within the range from 95 to 99%. 3 rotaviruses in northern Taiwan were possibly revolved through point mutation, genetic reassortment, and some of the strains were possibly imported from other countries. The information provided by this study will be beneficial for future vaccine development.
Subjects
rotavirus
epidemiology
phylogenetic analysis
vaccine
gene mutation
gene reassortment
VP7, VP4, NSP4, VP6 genes and amino acids
SDGs
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