Diagnosis of HCC by using a multi-photon fluorescence microscopy of spectrum, intensity, and lifetime
Date Issued
2010
Date
2010
Author(s)
Hsieh, Chien-Tai
Abstract
With multi-photon fluorescence spectra, multi-photon nonlinear microscopy and FLIM images, we successfully build up a bilirubin molecular imaging platform and imaged the metabolic disorders of bilirubins in HCC. By using an infrared femtosecond Cr:forsterite laser source with its wavelength away from two-photon resonance of most endogenous pigments, the distribution and amount of bilirubins can be semi-quantitively mapped over low-background autofluorescences. For non-tumor regions of liver tissues, the autofluorescences of bilirubins are strong and emitted from granular vesicles in hepatocytes. In poorly-differentiated HCC, the granular vesicles which emit fluorescence disappear and the intensity of multi-photon fluorescence is apparently reduced. If the hepatocytes have cholestasis, which is the signature of well-differentiated HCC, the fluorescence intensity were much higher than non-tumor part and the spectra showed an extra 800nm peak. These results indicate that, based on the multiphoton microscopy of autofluorescence spectra, intensities, and lifetimes, endogenous bilirubins are useful markers to indicate the presence and the staging of HCC. Without an extra labeling or chemical assays, disorder of bilirubins metabolisms in HCC can be sensitively detected.
Subjects
bilirubin
HCC
fluorescence
Type
thesis
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