Genetically engineered bacteria outer membrane vesicles coated small-molecule organic dye facilitates cDC1s-mediated antitumor immune cascade response for NIR-II tumor photoimmunotheranostics
Journal
Chemical Engineering Journal
Journal Volume
520
ISSN
1385-8947
Date Issued
2025-09
Author(s)
Chen, Yuhao
Geng, Xiaorui
Rao, Ganipisetti Hanumantha
Wei, Wei-Chih
Venkateswararao, Addanki
Liu, Tzu-Ming
Li, Yun
Ke, Pu Chun
Yuan, Zhen
Abstract
Although photothermal therapy (PTT) is a promising strategy for cancer treatment, the immunosuppressive tumor microenvironment promotes tumor progression and metastasis, limiting its effectiveness. To enhance adaptive antitumor immunity and improve post-PTT immunotherapy, genetically engineered bacteria outer membrane vesicles (OMVs)-modified small-molecule organic dye was constructed to serve as a nanophotosensitizer for NIR-II fluorescence/photoacoustic imaging-guided photo-immunotherapy. The developed nanophotosensitizer generated toxic hyperthermia under laser irradiation, promoting tumor antigen release. Meanwhile, the CD47nb-OMVs repolarized tumor-associated macrophages by blocking CD47-SIRRPα axis, relieving immunosuppressive tumor microenvironment. In addition, the present theranostic nanoplatform initiated tumor-resident type I conventional dendritic cells (cDC1s)-mediated antigen cross-presentation cascade, enhancing the downstream anti-tumor CD8+ T cell response. Furthermore, in vivo tests by using triple-negative breast cancer mouse model demonstrated that the novel NIR-II nanophotosensitizer effectively transformed the immunologically cold tumor microenvironment into a hot one, significantly improve survival rates, and inhibit distant metastases.
Subjects
CD47
Engineered bacteria
Immunotherapy
NIR-II optical imaging
OMV
Publisher
Elsevier BV
Type
journal article