Multi-photon resonance enhancement of third harmonic generation in human oxyhemoglobin and deoxyhemoglobin
Journal
Journal of Biophotonics
Journal Volume
3
Journal Issue
10-11
Pages
678-685
Date Issued
2010
Author(s)
Chang, C.-F.
Yu, C.-H.
Sun, C.-K.
Brown, C. Tom A.
Deckert, Volker
Sergeev, Alexer M.
Zheltikov, Aleksei M.
Abstract
Cancer cells require excessive oxygen and nutrition to support their rapid growth, so angiogenesis and decrease of blood oxygen are often associated with areas of cancer development. Current technologies for blood oxygen measurement, however, do not possess high spatial resolution and therefore cannot be used to detect small tumors in their early stage. In this paper, we studied the third harmonic generation (THG) spectra of oxy-and deoxyhemoglobin in the 1170-1365 nm region, which is strongly influenced by the multi-photon resonance effect, especially around the Soret transition band. Our spectroscopic results thus indicate the high potential of THG spectroscopic microscopy for oxygen depletion level measurement of a single red blood cell in vivo. ? 2010 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Subjects
Blood or tissue constituent monitoring; Multiharmonic generation; Nonlinear microscopy; Pulse oximetry
Other Subjects
Angiogenesis; Blood oxygen; Cancer cells; Cancer development; Current technology; Deoxyhemoglobin; High potential; High spatial resolution; In-vivo; Multiharmonic generation; Multiphotons; Nonlinear microscopy; Oxygen depletion; Pulse oximetry; Rapid growth; Single red blood cell; Small tumors; Third harmonic generations; Transition bands; Cells; Harmonic generation; Monitoring; Noninvasive medical procedures; Oximeters; Oxygen; Photons; Resonance; Scanning; Blood; deoxyhemoglobin; hemoglobin; oxygen; oxyhemoglobin; absorption; article; blood; human; metabolism; methodology; photon; spectroscopy; Absorption; Hemoglobins; Humans; Oxygen; Oxyhemoglobins; Photons; Spectrum Analysis
Type
journal article