家禽白血病 J 亞群病毒偵測方法之建立(2/2)
Date Issued
2001
Date
2001
Author(s)
王金和
DOI
892313B002151
Abstract
Subgroup J avian leukosis virus (ALV-J) causes
serious economic losses in commercial poultry
industry. Measuring group-specific (gs) antigen (Ag)
by ELISA has been used to identify chickens infected
with this virus. However, the inability of ELISA to
discriminate the gs Ags from endogenous and
exogenous origin has limited its usage. The purpose of
the present study was to find a method to discriminate
between endogenous and exogenous ALV gs Ag by
ELISA. The gs Ag and anti-ALV-J antibody in the
plasma samples from chickens at different ages in
three grand-parent farms were measured by ELISA.
Infected flocks were confirmed by RT-PCR using
different subgroup-specific primers. The results
indicated that the gs Ag of ALV-J-infected flocks
increased, but that of the uninfected flocks decreased
in young ages. The anti-ALV-J antibody of infected
flocks was higher and increased earlier than uninfected
flocks. In conclusion, measuring the gs Ag in blood at
the ages of 1 and 6 weeks by ELISA is suitable to
discriminate between endogenous and ALV-J infection.
This discovery is a valuable method for the detection
and control of ALV-J infection.
Subjects
ALV-J
ELISA
gs Antigen
RT-PCR
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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