The Lectin Receptor Kinase-VI.2 Is Required for Priming and Positively Regulates Arabidopsis Pattern-Triggered Immunity
Resource
PLANT CELL, 24(3), 1256-1270
Journal
The Plant Cell
Pages
1256-1270
Date Issued
2012
Date
2012
Author(s)
Singh, Prashant
Kuo, Yi-Chun
Mishra, Swati
Tsai, Chia-Hong
Chien, Chih-Cheng
Chen, Ching-Wei
Desclos-Theveniau, Marie
Chu, Po-Wei
Schulze, Birgit
Chinchilla, Delphine
Boller, Thomas
Abstract
Plant cells can be sensitized toward a subsequent pathogen attack by avirulent pathogens or by chemicals such as β-aminobutyric acid (BABA). This process is called priming. Using a reverse genetic approach in Arabidopsis thaliana, we demonstrate that the BABA-responsive L-type lectin receptor kinase-VI.2 (LecRK-VI.2) contributes to disease resistance against the hemibiotrophic Pseudomonas syringae and the necrotrophic Pectobacterium carotovorum bacteria. Accordingly, LecRK-VI.2 mRNA levels increased after bacterial inoculation or treatments with microbe-associated molecular patterns (MAMPs). We also show that LecRK-VI.2 is required for full activation of pattern-triggered immunity (PTI); notably, lecrk-VI.2-1 mutants show reduced upregulation of PTI marker genes, impaired callose deposition, and defective stomatal closure. Overexpression studies combined with genome-wide microarray analyses indicate that LecRK-VI.2 positively regulates the PTI response. LecRK-VI.2 is demonstrated to act upstream of mitogen-activated protein kinase signaling, but independently of reactive oxygen production and Botrytis-induced kinase1 phosphorylation. In addition, complex formation between the MAMP receptor flagellin sensing2 and its signaling partner brassinosteroid insensitive1-associated kinase1 is observed in flg22-treated lecrk-VI.2-1 mutants. LecRK-VI.2 is also required for full BABA-induced resistance and priming of PTI. Our work identifies LecRK-VI.2 as a novel mediator of the Arabidopsis PTI response and provides insight into molecular mechanisms governing priming.
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
15.pdf
Size
23.16 KB
Format
Adobe PDF
Checksum
(MD5):a295ee1ec5bc40b8a2acc7d64bdce6a7