Development of Activity-Based Probes for Imaging Human α- L -Fucosidases in Cells
Journal
Journal of Organic Chemistry
Journal Volume
80
Journal Issue
16
Pages
8458-8463
Date Issued
2015
Author(s)
Abstract
We have established a concise synthetic route relying on a key base-promoted epimerization step to synthesize two series of activity-based probes carrying a BODIPY fluorophore for α-l-fucosidase. The resulting probes were evaluated for labeling performance. The one utilizing an o-fluoromethylphenol derivative as the latent trapping unit was successfully applied for the first time to visualize and locate lysosomal α-l-fucosidase activity in human cells.
Type
journal article