Protein microarray using α-amino acids as metal tags on chips
Resource
Bioorganic & Medicinal Chemistry Letters 15 (5): 1413-1416
Journal
Bioorganic and Medicinal Chemistry Letters
Journal Volume
15
Journal Issue
5
Pages
1413-1416
Date Issued
2005
Author(s)
Abstract
Procedures for synthesizing alpha-amino acids on a chip for coordination with transitional metal ions and a His-Tagged protein have been successfully developed as a stable protein microarray. Using the recombinant His-Tagged 3CL-protease (3CLpro) as a model for attachment to chips containing D-/L-Glu, Asp, Orn, Ser via different transitional metal ions, it was found that the Orn chip was the best of affinity binding and stability by which Zn2+ was the best metal ion for affinity while Co2+ was the best metal ion for stability. Thus, this protein microarray can be alternatively used as a high throughput screening method for rapid detection against SARS CoV 3CLpro and/or efficient purification of other Tagged proteins.
Type
journal article
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