綠竹筍生長與老化機制之探制及相關功能基因之利用─綠竹及綠竹筍老化過程中抗氧化機制之角色及其應用性探討(2/2)
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
DOI
922317B002014
Abstract
Activity staining and isozymes identification of SODs in the different part of bamboo
shoot and leaf (Bambusa oldhamii) indicated that primary SOD activity in green
bamboo shoots was composed of MnSOD and CuZnSOD activities. As compared to
SOD isozymes from over one hundred plants, green bamboo’s CuZnSOD and
MnSOD showed great difference in native PAGE activity staining. (Pan et al., 1999),
reflecting green bamboo’s CuZnSOD gene complexity, therefore inferring possible
molecular structures and biochemical activities specificity in green bamboo’s SOD
isozymes. We have completed the SODs activity and mRNA gene expression assay by
Northern blotting using different tissues from various growth stages of green bamboo
and suspension cell treated with UV-B, intensive light, heat, cold and ABA. We show
that there are 4 to 5 CuZnSOD and MnSOD genes, respectively according to the
Southern blotting data. We cloned 4 different full length CuZnSOD cDNAs and 2 full
length MnSOD cDNAs. The 3'-RACE sequence results from MnSOD shows 5
different 3’-UTRs sequences, which is in agreement with the data that green bamboo,
has more CuZnSOD and MnSOD gene families. Now we have conducted 3
GST-CuZnSOD and 2 GST-MnSOD recombinant proteins expression in E. coli and
acquired different native PAGE activity stain patterns. In the future, we will express
these genes in Arabidopsis and analyze these recombinant protein’s biochemical
activities in vitro.
Subjects
activity stain
oxidative stress
recombinant protein
Publisher
臺北市：國立臺灣大學植物科學研究所
Type
journal article
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