Evaluation of hybridization conditions for spotted oligonucleotide-based DNA microarrays.
Resource
Molecular Biotechnology,29(3),221-224.
Journal
Molecular Biotechnology
Journal Volume
29
Journal Issue
3
Pages
221-224
Date Issued
2005-03
Date
2005-03
Author(s)
Tsai, M.H.
Yan, H.
Chen, X.
Chandramouli, G.V.R.
Zhao, S.
Coffin, D.
Coleman, C.N.
Mitchell, J.B.
Chuang, E.Y.
Abstract
We compared different hybridization conditions of oligonucleotide-based DNA microarray to acquire optimized and reliable microarray data. Several parameters were evaluated at different hybridization conditions, including signal-to-background (S:B) ratios, signal dynamic range, usable spots, and reproducibility. Statistical analysis showed that better results were obtained when spotted, presynthesized long oligonucleotide arrays were blocked with succinic anhydride and hybridized at 42 degrees C in the presence of 50% formamide.
File(s)![Thumbnail Image]()
Loading...
Name
903.pdf
Size
23.29 KB
Format
Adobe PDF
Checksum
(MD5):b3693075f18d699de5a3dfcca5dc402d