The effects of growth inhibitory peptide on follicular thyroid cancer cell growth, migration, and invasion
Journal
Tumori
Journal Volume
96
Journal Issue
3
Pages
448-451
Date Issued
2010
Author(s)
Abstract
Aims and background. Thyroid cancer is the most common endocrine neoplasm worldwide. Although differentiated thyroid cancers are associated with a favorable survival, the prognosis worsens dramatically for patients with distant metastasis. Metastases from follicular thyroid carcinoma (FTC) occur earlier and are more aggressive than those from papillary thyroid carcinoma. For FTC that is resistant to radioactive iodine, new treatments are urgently needed. Human alpha-fetoprotein (HAFP) is a tumor-associated fetal protein that has been demonstrated to regulate tumorigenesis. Growth inhibitory peptide (GIP), a synthetic 34-mer peptide isolated from the third domain of HAFP, has been shown to have antitumor growth ability in various human cancers. However, the effects of GIP in FTC have not yet been studied. The aim of this study was to investigate the antitumor ability of GIP in FTC. Methods and study design. Using both PBS and GIP control peptide as a negative control, the antiproliferative activity of GIP in the WRO human FTC cell line was determined using a tetrazolium-based colorimetric assay. In addition, cell migration and invasion assays were used to measure tumor metastasis inhibition effects in vitro. Results. GIP did not inhibit WRO cell proliferation in a time- or dose-dependent manner. However, in WRO cells treated with GIP for 4 days, migration was significantly inhibited at concentrations of 50 and 100 μM (33.3% and 19.5%, respectively; both P <0.05). Cell invasion was also significantly inhibited at 50 and 100 μM (67.1% and 39.0%, respectively; both P <0.05). Conclusions. Although GIP failed to suppress FTC cell growth, it effectively interrupted both FTC cellmigration and invasion abilities in vitro. Further validation in an animal model and elucidation of the underlying mechanisms will be required. GIP may potentially serve as an anti-FTC metastasis agent aiding current chemotherapy regimens.
Subjects
Cell invasion; Cell migration; Cell proliferation; Follicular thyroid cancer; Growth inhibitory peptide; Metastasis
SDGs
Other Subjects
alpha fetoprotein; antimetastatic agent; growth inhibitory peptide; radioactive iodine; synthetic peptide; unclassified drug; antineoplastic activity; article; cancer cell; cancer invasion; cell growth; cell migration; cell viability; colorimetry; controlled study; dose time effect relation; human; human cell; metastasis inhibition; peptide synthesis; thyroid carcinoma; thyroid follicle cell; thyroid follicular carcinoma
Type
journal article