Revealing the role of nitrate on sulfide removal coupled with bioenergy production in Chlamydomonas sp. Tai-03: Metabolic pathways and mechanisms
Journal
Journal of Hazardous Materials
Journal Volume
399
Date Issued
2020
Author(s)
Abstract
Recently, simultaneous sulfide removal and bioenergy production by microalgal treatment have attracted growing attention. However, the response of nitrogen metabolism to the sulfide-removal process has yet to be explored. Here, variable levels of sulfide could be completely removed by Chlamydomonas sp. Tai-03 under both high and low nitrate conditions in synthetic wastewaters. The highest sulfide removal rate of 5.56 mg-S L−1 h−1 was achieved with the addition of 100 mg L−1 sulfide in the presence of high nitrate. Meanwhile, sulfide was chemically oxidized to sulfate and then ingested by microalgae. Interestingly, sulfide-removal efficiency critically depended on nitrate concentration. Sulfide can also enhance the ability of microalgae to assimilate nitrogen. Based on the analysis of sulfur- and nitrogen-related metabolic profiling, serine as a precursor decreased by 94 % under low levels of nitrate, which induced the significant inhibition of cysteine and methionine biosynthesis. The results indicated that nitrogen source played a critical role in the sulfur cycle because of the positive relationship between the aforementioned metabolic processes and nitrate concentration. Additionally, sulfide can improve lipid and carbohydrate productivity under high levels of nitrate. This study enhances our understanding of the mechanisms underlying the simultaneous removal of sulfide and alternative bioenergy production. © 2020 Elsevier B.V.
Subjects
Bioenergy production; Microalgae; Nitrate; Sulfide removal; Sulfur metabolomics
Other Subjects
Algae; Amino acids; Biochemistry; Metabolism; Microorganisms; Nitrates; Nitrogen removal; Sulfur; Bioenergy productions; Metabolic pathways; Metabolic profiling; Methionine biosynthesis; Nitrate concentration; Nitrogen metabolism; Simultaneous removal; Synthetic waste water; Sulfur compounds; acetyl coenzyme A; carbohydrate; cysteine; deferiprone; glucose 1 phosphate; glucose 6 phosphate; glycerophosphate; lipid; methionine; nitrate; nitrogen; phosphate; reactive oxygen metabolite; serine; sulfate; sulfide; sulfur; nitric acid derivative; sulfide; bioenergy; carbohydrate; concentration (composition); inhibition; metabolism; microalga; nitrate; pollutant removal; sulfide; Article; bioenergy; carbohydrate analysis; carbon metabolism; chemical analysis; Chlamydomonas; clinical evaluation; concentration process; metabolic fingerprinting; metabolic regulation; nitrogen cycle; nitrogen metabolism; nonhuman; sulfur cycle; waste water management; bioreactor; metabolism; Chlamydomonas; Bioreactors; Chlamydomonas; Metabolic Networks and Pathways; Nitrates; Nitrogen; Sulfides
Type
journal article