OXIDATIVE DNA DAMAGE ESTIMATED IN RELATION TO INDOOR CARBON DIOXIDE, VOLATILE ORGANICS AND TOBACCO SMOKE EXPOSURES
Resource
EPIDEMIOLOGY v.16 n.5 pp.S42
Journal
EPIDEMIOLOGY
Journal Volume
v.16
Journal Issue
n.5
Pages
S4-2
Date Issued
2005
Date
2005
Author(s)
MA, YEE-CHUNG
LIN, JIA-MING
SUNG, FUNG-CHANG
Abstract
Introduction: Carbon dioxide (CO2), volatile organic compounds (VOCs) and environmental tobacco smoke (ETS) are common indoor air pollutants generated from human activities in buildings. This study investigated urinary 8- hydroxydeoxyguanosine (8-OHdG) as an effective biomarker of DNA damage associated with indoor air pollutants among office building occupants. Methods: With consents and indoor CO2 and VOCs measured simultaneously, urinary 8-OHdG and cotinine were determined for 344 non-smokers and 45 smokers recruited from 8 air-conditioned office buildings in Taipei. CO2and total VOCs were measured with portable monitors (Q- TRAK IAQ Model 8551, TSI Incorporated, Shoreview, MN, U.S.A, and PGM-7240, RAE SYSTEMS, California, U.S.A., respectively ). Urinary 8-OHdG and cotinine were determined by enzyme- linked immunosorbent assay (ELISA) and barbituric acid assay , respectively. Results: There were large variations in concentrations of CO2 (median= 901 ppm; range=“530~2794” ppm) and total VOCs (median= 190 ppb; range= 1~55733 ppb), and urinary cotinine levels (median= 2135 mg/g creatinine; range= 486~8984 mg/g creatinine). The average 8-OHdG levels were approximately two times higher in smokers than in non- smokers for both females (5.06 vs. 11.3 mg/g creatinine) and males (5.08 vs.10.6 mg/g creatinine). The multivariate logistic regression analysis showed that, comparing with individuals with CO2 exposure at the lowest quartile, the odds ratios (ORs) of having the urinary 8-OHdG levels greater than the overall median value, 4.99 mg/g creatinine, were 7.66 (95% confidence interval (CI) = 3.08–19.1) for individuals with the exposure at the third quartile level, and 14.3 (95% CI = 14.13–49.7) for those at the highest quartile level. Smokers were 6.33 times (95% CI = 1.44–27.8 ) more likely than never smokers to have such an elevated urinary 8-OHdG level. There was no significant association between VOCs and 8-OHdG excreted. Conclusion: The indoor CO2 and tobacco smoke exposure contribute independently to elevated DNA damage, using urinary 8-OHdG levels as a sensitive biomarker.
Type
journal article