High Resolution Genetic Mapping and Candidate Gene Identification of the SLP Locus Controlling Glume Development in Rice
Date Issued
2009
Date
2009
Author(s)
Wang, Sheng-Shan
Abstract
Stunted lemma/palea (slp) is a sodium azide-induced rice mutant displaying dwarf, shorten panicle length, seriously degenerated lemma/palea, and sterility. Cloning and characterization of the SLP locus may shed light on the molecular basis and evolutionary history of glume development in grass. Genetic analysis confirmed that slp phenotype was controlled by a single gene with pleiotropic effect on plant height and several traits related to inflorescence architecture. The slp mutant allele was incomplete dominant to the normal allele for most of affected traits but plant height. Genetic mapping based on the genotypes of 82 polymorphic SSR markers in 42 F2 plants which was derived from the cross between SLP/slp (Oryza sativa subsp. japonica) and Taichung native 1 (O. sativa subsp. indica), suggested that the SLP locus was mapped between markers RM23477 and RM23652 in the distal region of the long arm chromosome 8. Additional 134 F2 individuals were used to delimit the SLP locus in a smaller chromosomal region between marker RM447 and D275. A high-resolution genetic map surrounding the SLP locus was developed using 8 recombinant F2 individuals screened from 982 F2 plants. The SLP locus was eventually delimited in a 46.4 kb genomic region containing three putative genes, OsSPL16, OsMADS45 (also known as OsMADS7)and OsMADS37. Detection of single nucleotide polymorphism and comparisons of gene expression level between the SLP/SLP and slp/slp genotypes for three candidate genes suggested that a missense mutation at the sixth amino acid of the OsSPL16 protein was likely responsible for the slp mutant phenotypes.
Subjects
glumes
rice
genetic mapping
mutant
Type
thesis
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