Effect of ethylene inhibitors on the emasculation-induced senescence of Phalaenopsis floret
Date Issued
2011
Date
2011
Author(s)
Liu, Ying-Chin
Abstract
Phalaenopsis (Phalaenopsis spp.) is a climacteric flower which produces a large amount of ethylene during aging. Phalaenopsis floret is sensitive to ethylene and ethylene is considered as the main factor affecting the quality and vase-life of the flower. Silver thiosulfate (STS), aminooxyacetic acid (AOA), aminoethoxyvinylglycine (AVG) and Co2+ are well-known ethylene inhibitors. Removal of the pollina and anther cap from an orchid flower, which is called emasculation, had been shown to hasten the upsurge of ethylene and was accompanied by flower senescence in various orchid species. By taking advantage of the rapid ethylene synthesis after emasculation, a simple evaluation system, which used single Phalaenopsis floret held in 7 cm long flasks, was developed for studying the effectiveness of ethylene inhibitors on the senescence of Phalaenopsis floret.
Continuous treatments of 0.4 mM STS, 0.5 mM AOA, 0.5 mM AVG and 2mM CoCl2 were able to maintain the fresh weight, delay the emasculation-induced senescence and inhibit the ethylene production of ‘Phal. Crystal Veil’ Phalaenopsis florets. For ‘Dtps. Han Ben’s Girl’ and ‘Dtps. Sogo Yukidian’ Phalaenopsis florets, 0.4 mM STS, 0.5 mM AOA, 0.5 mM AVG and 4 mM CoCl2 were found effective. Among these treatments, 0.5 mM AOA was the most effective one; it significantly extend the vase life of ‘ Phal. Crystal Veil’, ‘Dtps. Han Ben’s Girl’ and ‘Dtps. Sogo Yukidian’ florets 3, 6 and 7 folds as compared with the control. Mixing two effective compounds in continuous treatment, did not result in better performance than single-compound treatment, and 0.5 mM AOA remained as the most effective treatment. For normal, un-emasculated ‘Phal. Crystal Veil’ Phalaenopsis florets, 0.4 mM STS was most effective, followed by 0.5 mM AOA; both treatments extended the vase life more than three folds than control.
Pulsing treatment of ‘Dtps. Han Ben’s Girl’ floret with all the previous compounds for 6 hours, effectively delayed the senescence of emasculated florets. Doubling the dosage further extended the vase life and maintained the fresh weight of ‘Dtps. Han Ben’s Girl’ floret. Among the treatments, 0.8 mM STS, 1.0 mM AOA and 8 mM CoCl2 were most effective; the vase life was extended 2 – 3 fold of the control. Mixing two effective compounds for pulsing treatment were more effective than single compounds. Pulsing with 0.4 mM STS plus 0.5 mM AOA resulted in nine to ten folds increase in vase life than the control. For un-emasculated ‘Dtps. Han Ben’s Girl’ florets, pulsing with mixed inhibitors for 6 hours also maintained the fresh weight longer than the control. Pulsing with 0.4 mM STS plus 0.5 mM AOA and 0.4 mM STS plus 0.5 mM AVG were most effective; the vase life were extended two to three folds compared to the control.
The effective compounds were applied to the whole cut inflorescence of ‘Dtps. Han Ben’s Girl’ during a simulate shipment at 20℃ for 3 days, and the results showed that pulsing with of 0.4 mM STS plus 0.5 mM AOA before shipment for 6 hours, and continuous treatment of 0.5 mM AOA during shipment could extend the vase life of the cut flower more than 2 folds than the control.
Subjects
ethylene
ethylene inhibitor
Phalaenopsis
emasculation
senescence
Type
thesis
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