Stimulation of Breast Milk Secretion by Yeast Fermented Cereal
Date Issued
2014
Date
2014
Author(s)
Wu, Jhih-Yu
Abstract
Mother milk plays an important role for a baby; lactation is also important for livestock. Therefore, many people want to know how to increase the milk quantity and quality. Especially, every mother wants to avoid being lack of mother milk. It was said traditional food fermented glutinous rice was good for stimulating lactation for a mother. Yeast fermented product or live yeast were also fed to animals to stimulation of lactation but scientists guess that the reasons to increase milk secretion is the yeast influenced the microflora of rumen so the digestibility become better. Our research aim to understand whether the yeast fermented products will directly influence the mammary epithelial cell and further influence the lactation. Because the solid state fermentation product is easy to dry so the research chose the solid state fermentation and used the soybean meal and wheat as material that they are cheap and easy to get. The strains used for fermentation were Aspergillus oryzae, Saccharomyces cerevisiae, Lactobacillus acidophilus and Lactobacillus casei. Aspergillus oryzae was used for aerobic fermentation; Saccharomyces cerevisiae was used for further fermentation and producing metabolic product. Finally, Lactobacillus acidophilus and Lactobacillus casei were used to lower down the pH value in order to have longer shelf time. In the research, there were four different procedures to fermentation. First, the material was aerobic fermented by A. oryzae for elevating the utilization for next step anaerobic fermentation. Then, use Saccharomyces cerevisiae anaerobic fermentation for 0 h, 24 h, 48 h and 72 h. Second, as the first procedure but add Lactobacillus acidophilus and Lactobacillus casei to anaerobic fermentation for one more day. Third, use only Saccharomyces cerevisiae to do anaerobic fermentation. Last, use Saccharomyces cerevisiae and Lactobacillus acidophilus and Lactobacillus casei do anaerobic fermentation. The results showed the Aspergillus oryzae was toxic for mammary epithelial cell. When the material was fermented only by yeast or yeast, the cell would proliferate. When treat with high concentration (500 μg/ml~2000 μg/ml) the cell will proliferation. Further analysis for beta casein expression, the results show using the extract fermented only by yeast for 48 and 72 h and concentration of 500 μg/ml to 2000 μg/ml will increase beta casein expression. The result showed the yeast fermented product could stimulate the proliferation and beta casein expression of epithelial cell and the procedure of fermentation also influenced the results. Use only aerobic fermentation by yeast or yeast and lactic acid is a suitable procedure for stimulation of lactation.
Subjects
酵母菌
乳腺上皮細胞
固態發酵
Beta-酪蛋白
Type
thesis
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