柑桔黃龍病菌系統之生物性與分子性鑑別(Ⅱ)
Date Issued
2003
Date
2003
Author(s)
DOI
912313B002369
Abstract
Citrus huanglongbing (HLB), previously called greening, is a serious citrus disease in Asia, eastern and southern Africa.
It is caused by
Candidatus
Liberibacter asiaticus (Las), a phloem-limited, nonculturable bacterium transmitted by the
Asian citrus psyllid (
Diaphorina citri
) in Asia. A PCR-based assay was developed for monitoring Las in vector psyllids
using a rapid DNA extraction from psyllid bodies and PCR amplification. The entire procedure for Las detection in
psyllids can be completed within 5 h. Using this method, Las can be accurately detected in psyllid adults as well as nymphs
in different instar stages. The assay is sensitive enough for Las detection in single-psyllid extract from adult, fifth, fourth
and third instars. In a transovarial transmission experiment, Las was not detected in eggs or in offspring produced by
Las-carrying psyllid females. In a retention test, the Las-carrying psyllids remained Las-positive for 12 weeks after they
were moved to common jasmine orange, a Las-immune plant. From these experimental results it was concluded that Las
persists in the Asian citrus psyllid vector, but is not transovarially transmitted by the vector. These data help in understanding
epidemiological characteristics of Las and psyllids in citrus HLB.
Subjects
Asian citrus psyllid
Candidatus
Liberibacter asiaticus
Liberibacter asiaticus
citrus huanglongbing
mycoplasma
phytoplasma
Publisher
臺北市：國立臺灣大學植物病理與微生物學系暨研究所
Type
other
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