Evaluation of biodegradable elastic scaffolds made of ionomer-based waterborne polyurethane for tissue engineering
Date Issued
2014
Date
2014
Author(s)
Tsai, Meng-Chao
Abstract
Biodegradable polyurethane (PU) was synthesized by a green and sustainable water-based process. The process rendered homogenous PU nanoparticles (NPs). Spongy PU scaffolds in large dimension were obtained by freeze-drying the PU NP dispersion. The spongy scaffolds were examined in terms of the porous structure, wettability, mechanical properties, degradation behavior, and degradation products. The capacity as cartilage tissue engineering scaffolds was evaluated by growing chondrocytes and mesenchymal stem cells (MSCs) in the scaffolds. Scaffolds made from PU dispersion had excellent hydrophilicity. The scaffolds had high porosity and water absorption. Examination by micro-computed tomography confirmed that PU scaffolds had good pore interconnectivity. The degradation rate of the scaffolds immersed in phosphate buffered saline was much faster than that in papain solution or in deionized water at 37 oC. The biodegradable PU appeared to be degraded via the cleavage of ester linkage, judging from the degradation products. The intrinsic elastic property of PU and the gyroid-shape porous structure of the scaffolds may have accounted for the outstanding strain recovery (87 %) and elongation behavior (257 %) of the PU scaffolds, compared to conventional poly(D,L-lactide) (PLA) scaffolds. Chondrocytes were effectively seeded in PU scaffolds without pre-wetting. They grew better and secreted more glycosaminoglycan in PU scaffolds vs. PLA scaffolds. Human MSCs showed greater chondrogenic gene expression in PU scaffolds than in PLA scaffolds after induction. Based on the favorable hydrophilicity, elasticity, and regeneration capacities, the novel biodegradable PU scaffolds may be superior to the conventional biodegradable scaffolds in cartilage tissue engineering applications.
Subjects
水性生物可降解聚胺酯
支架
降解
彈性
軟骨
Type
thesis
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