Alleviation of Insulin Resistance and Improvement in Carbohydrate Metabolism in TNF-α Treated Mouse Liver FL83B Cells by Pink Wax Apple Fruit Water Extract
Date Issued
2011
Date
2011
Author(s)
Chang, Chiao-Li
Abstract
Diabetes mellitus (DM) is a chronic disease associated with carbohydrate metabolism and is caused by a deficiency in insulin secretion or by the ineffectiveness in insulin action. Around 95% of the diabetic incidences belong to type 2 diabetes, of which, the main cause is the ineffectiveness in insulin action. Literatures confirmed the anti-diabetic effect of Myrtaceae plants. In a previous study, we found the high polarity fraction from pink wax apple aqueous extract (FPWE) may improve glucose uptake in insulin resistant mouse hepatocytes. In the present study, mouse FL83B hepatocytes were treated with tumor necrosis factor-α (TNF-α) for induction of insulin resistance. FPWE was used for investigating the expressions of insulin signal protein and glycolysis associated protein in insulin resistant hepatocytes. The effect of FPWE on inflammation in insulin resistant FL83B was also evaluated.
The results show that FPWE promotes the phosphorylation of insulin receptor tyrosyl and the expressions of insulin receptor substrate-1 and the insulin signal associated proteins, including insulin receptor, phosphatidylinositol-3 kinase (PI3K), Akt/PKB, glycogen synthase (GS) and glucose transporter-2 (GLUT-2). In the aspect of carbohydrate metabolism, the results revealed that FPWE increases the expression of hexokinase (HXK), glucose-6-phosphate dehydrogenase (G6PDase), phosphofructokinase (PFK) and aldolase. In the respect of inflammation, FPWE decreases phosphorylation of JNK and the protein expressions of ERK, NF-κB and COX-2 in the resistant FL83B cells.
In conclusion, FPWE may improve the glucose uptake in insulin resistant mouse FL83B hepatocytes via the reduction of inflammation and the incensement of insulin sensitivity.
Subjects
diabetes mellitus
insulin resistance
young pink wax apple fruit aqueous extract
carbonhydrate metabolism
inflammation
SDGs
Type
thesis
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