The effects of advanced glycation end products on sexual hormonal secretion in vivo and in vitro
Date Issued
2015
Date
2015
Author(s)
Wu, Kun-Hsuan
Abstract
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age. PCOS is characterized by menstrual cycle disorders, hyperandrogenism and polycystic ovary morphology. No single etiologic factor fully accounts for the spectrum of abnormalities in the polycystic ovary syndrome. Hormonal abonormality and insulin resistance may be associated with the pathogenesis of PCOS in current study. Granulosa cells are specific endocrine cells in ovaries which regulate menstrual cycle and the development of follicles by secreting progesterone and conversion of androgen to estradiol. Advanced glycation end products (AGEs) are known that can spark the development of inflammation and diabetes complications. Research has shown that serum AGEs level was elevated in women with PCOS. Other studies have indicated that consumption of high-AGEs diet can affect hormone expression and enhance oxidative stress. In the present study, we prepared different AGEs by mixing methylglyoxal (MG), glyceraldehyde (GA), glyoxal (GO), glycolaldehyde (GOA) and glucose (GLU) with bovine serum albumin. Human granulosa cell lines (KGN) and human primary granulosa cells are used to investigate the effects of AGEs on hormonal secretion and the proliferation of ovarian cells. Further effect was confirmed by in vivo study. It was observed that five different AGEs treatments significantly inhibit the proliferation of granulosa cells and the dose-dependence was existed. GOA-BSA has the most significant inhibitory effect. The parts of hormonal secretion, MG-BSA, GA-BSA, GO-BSA significantly inhibit progesterone secretion by different stimulators. MG-BSA can significantly increase insulin resistance, serum aspartate transaminase level and inhibit progesterone release in vivo. In conclusion, AGEs can affect granulosa cells proliferation, progesterone secretion and insulin resistance which are risk factors of PCOS.
Subjects
Polycystic ovary syndrome (PCOS)
granulosa cell
advanced glycation end product (AGEs)
SDGs
Type
thesis
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