Neutralization of White Spot Syndrome Virus by Monoclonal Antibodies against Viral Envelope Proteins
Resource
Taiwania,49(三)
Journal
Taiwania
Journal Volume
49
Journal Issue
三
Pages
-
Date Issued
2004
Date
2004
Author(s)
DOI
246246/2006121215550986
Abstract
Two monoclonal antibodies (MAbs) recognizing envelope proteins of the white spot
syndrome virus (WSSV), 6E1 against VP28 and 3E8 against VP19, were applied to demonstrate their
neutralizing ability to this virus by using both in vitro and in vivo assays. Mixtures of MAb 6E1 with
virus filtrate were inoculated into the primary explant monolayer culture derived from the lymphoid
Oka organs of Penaeus monodon. MAb was likely to neutralize the infectivity of virus to monolayer
since cytopathic effects were apparently blocked in experiment group. WSSV was titrated using
Blue-Cell ELISA and the neutralizing index was calculated to be 6.90 for 6EI and 5.83 for 3E8.
Neutralized virus fluids injected intramuscularly into post larvae of P. monodon. The shrimp in the
positive control, which were injected with WSSV only showed an increasing mortality and a 100%
mortality was reached at day 34, whereas no shrimp died in the negative control. The mortality for
6E1 was 6.7% and for 3E8 was 13.3%. These results suggest that MAbs recognizing the WSSV
envelope proteins could neutralize viral infectivity to both cultured cells and shrimp.
Subjects
White spot syndrome virus
WSSV
MAbs
Neutralization
Primary culture
Publisher
臺北市：國立台灣大學生態學與演化生物學研究所
Type
other
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