Molecular cloning of a Poria cocos protein that activates Th1 immune response and allays Th2 cytokine and IgE production in a murine atopic dermatitis model
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
62
Journal Issue
13
Pages
2861-2871
Date Issued
2014
Author(s)
Abstract
Edible fungus Poria cocos (Schw.) Wolf is a cooking material that has myriad health benefits. However, its active constituents have not been well-defined. We previously purified an immunomodulatory protein, PCP, from P. cocos and described its biochemical features and its ability to activate primary macrophage via TLR4. In this study, we cloned the gene of PCP and demonstrated its ability to activate Th1 response in cell cultures and in mice. The complete cDNA sequence of PCP consisted of 807 bp, which included a 579 bp coding sequence that encoded 194 amino acids. With the addition of co-stimulatory CD3/CD28 signals, PCP significantly increased the surface expression of CD44 and CD69 on effector T cells. PCP could also up-regulate T-bet and STAT4 expressions and IFN-γ and IL-2 secretions. Oral administration of PCP suppressed the production of both total and OVA-specific IgG1 in serum and enhanced the amounts of serum and OVA-specific IgG2a and Th1-related cytokine production in BALB/c splenocytes. In addition, oral administration of PCP significantly reduced IL-4 and IgE expressions in a murine model of atopic dermatitis. In conclusion, these results provide evidence that PCP could regulate mammalian immune cells and reveal their pharmaceutical potential in developing therapeutic strategies against Th2-mediated immune disorders. ? 2014 American Chemical Society.
Subjects
atopic dermatitis; fungal immunomodulatory protein; molecular cloning; Poria cocos; Th1 immune response
SDGs
Other Subjects
Amino acids; Body fluids; Cell culture; Cloning; Immune system; Mammals; Proteins; Amino acids; Body fluids; Cell culture; Cloning; Dermatitis; Genetic engineering; Immune system; Mammals; Proteins; Atopic dermatitis; Immune response; Immunomodulatory; Molecular cloning; Poria cocos; T-cells; T-cells; Fungi; Mammalia; Murinae; Mus; Poria cocos; fungal protein; gamma interferon; immunoglobulin E; interleukin 2; interleukin 4; amino acid sequence; animal; article; atopic dermatitis; Bagg albino mouse; chemistry; female; genetics; human; immunology; molecular cloning; molecular genetics; mouse; nucleotide sequence; Poria; Th1 cell; Th2 cell; Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Dermatitis, Atopic; Female; Fungal Proteins; Humans; Immunoglobulin E; Interferon-gamma; Interleukin-2; Interleukin-4; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Poria; Th1 Cells; Th2 Cells
Type
journal article