The Mechanism Study Underlying the Anti-oxidant and Anti-inflammation Activities of Ergostatrien-3β-ol from Antrodia camphorata Using Ischemia and Reperfusion Injury Rat Model
Date Issued
2014
Date
2014
Author(s)
Hsu, Shih-Min
Abstract
Antrodia camphorate has been considered as one of most efficient and precious Chinese herbal medicines, and it has been used as remedy for curing food poisoning, diarrhea, stomachache, hypertension, itchy skin, and liver cancer. Recently, liver protecting characteristic of Antrodia camphorata was believed as the most important bioactivity. Ergostatrien-3β-ol (EK100), which exists in both fruiting body and mycelia, is one of compounds with high liver-protecting property； however, investigation for this compound is scarce. In the present research, animal model with hepatic ischemia and reperfusion injury (IR injury) was created, and EK100, which is asserted to possess liver-protecting and anti-inflammatory effects, was used for decreasing live damages resulted from IR injury. Clinically, IR injury occurs in patients with myocardial infraction, stroke, or large trauma, in which tissue is injured irreversibly by deficient supply of oxygen due to blood flow blocking, and harmed again by violet inflammatory response brought from fast and massive accumulation of reactive oxygen species after blood is resupplied. In our animal study, EK100 decreased ALT and AST, which is mainly come from liver injury, and it also lowered the proinflammatory cytokines such as TNF-α, IL-6, as well as IL-1. In addition, EK100 significantly reduced the mRNA expression of iNOS, and increased SOD, which is an antioxidant enzyme in liver. A further investigation in anti-inflammatory and antioxidant mechanisms of EK100 was conducted by cell model. EK100 improved the translocation of Nrf2 into nuclei, activating the downstream genes of HO-1 and NQO1. The most efficient dosage and effect time were observed at 10 μM and 17 hours.
Subjects
肝臟缺血再灌流損傷(hepatic ischemia and reperfusion injury；hepatic IR injury)
抗氧化(antioxidation)
抗發炎(anti-inflammation)
Nuclear factor erythroid 2-related factor 2 (Nrf2)
HO-1 (Heme oxygenase-1)
SDGs
Type
thesis
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