Observation of the Death Process of Cancer Cell with Optical Coherence Tomography
Date Issued
2016
Date
2016
Author(s)
Chen, Shih-Yang
Abstract
The use of a high-resolution optical coherence tomography (OCT) system with the operation wavelength around 800 nm to scan SCC4 cancer cells under different laser illumination conditions is demonstrated. The cancer cells are incubated with Au nanorings (NRIs), which are either linked with the photosensitizer, AlPcS, or not, for them to be up-taken by the cells. Two Au NRI samples of different geometries for inducing localized surface plasmon (LSP) resonance around 1310 and 1064 nm are used. Four different lasers are utilized for illuminating the cells under OCT scanning, including 1310-nm continuous (cw) laser, 1064-nm cw laser, 1064-nm femtosecond (fs) laser, and 660-nm cw laser. The 1310- and 1064-nm cw lasers mainly produce the photothermal effect through the LSP resonance of Au NRIs for damaging the observed cells. Besides the photothermal effect, the 1064-nm fs laser can produce strong two-photon absorption through the assistance of the LSP resonance of Au NRI for exciting AlPcS to effectively generate singlet oxygen and damage the observed cells. The 660-nm laser can excite AlPcS through single-photon absorption for generating singlet oxygen and damaging the observed cells. With the strong photothermal effect, the observed cells can be killed through the process of necrosis. Through the generation of singlet oxygen, the cell membrane can be preserved and the interior substances are solidified to become a hard body of strong scattering. In this situation, the cells are killed through the apoptosis process. Illuminated by the 660-nm cw laser, a process of interior substance escape is observed through high-speed OCT scanning.
Subjects
Cell Death Process
Optical Coherence Tomography
SDGs
Type
thesis
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