Functional analysis of EBV in nasopharyngeal carcinoma cells
Journal
Laboratory Investigation
Journal Volume
83
Journal Issue
6
Pages
797-812
Date Issued
2003
Author(s)
Abstract
A membrane invasion culture system was used to study the ability of EBV to enhance invasion and migration of nasopharyngeal carcinoma (NPC) cells. Semi-reverse transcriptase-PCR analysis of matrix proteinases and angiogenic factors from EBV-infected, or EBV-positive (EBV+), cells demonstrated different degrees of elevated gene expression. In our animal model, EBV+ tumors grew faster and larger than EBV-free, or EBV-negative (EBV-), tumors and also had clonal EBV terminal repeat sequences. Double-localization of EBV and certain host proteins in EBV+ tumors and biopsy specimens demonstrated that EBV up-regulates host genes only in cells that express those genes but not in cells that do not express them. Double-localization of EBV and host genes in NPC biopsy specimens all showed EBV-tumor cells expressing those host genes. Our data strongly suggest that EBV infection enhances progression of NPC tumor growth. They do not rule out a role for EBV infection in the induction and early promotion of NPC development. Unidentified factors may also enhance NPC tumor growth independent of the effects of EBV.
SDGs
Other Subjects
angiogenic factor; matrix metalloproteinase; animal model; animal tissue; article; cancer cell culture; carcinoma cell; cell growth; cell invasion; cell migration; cell structure; controlled study; Epstein Barr virus; gene expression; gene expression regulation; human; human cell; long terminal repeat; mouse; nasopharynx carcinoma; nonhuman; priority journal; protein localization; reverse transcription polymerase chain reaction; SCID mouse; solid tumor; tumor growth; tumor promotion; virus carcinogenesis; virus detection; virus genome; virus infection
Publisher
Lippincott Williams and Wilkins
Type
journal article