OXIDATIVE DNA DAMAGE ESTIMATED BY URINARY 8-HYDROXYDEOXYGUANOSINE: INFLUENCE OF TAXI DRIVING, SMOKING AND ARECA CHEWING
Resource
CHEMOSPHERE v.52 n.7 pp.1163-1171
Journal
CHEMOSPHERE
Journal Volume
v.52
Journal Issue
n.7
Pages
1163-1171
Date Issued
2003
Date
2003
Author(s)
SUNG, FUNG-CHANG
Abstract
Nitrogen oxides (NO.) and polycyclic aromatic hydrocarbons are common air pollutants generated from automobile exhaust and cigarette smoke. This study was to investigate urinary 8 -hydroxydeoxyguanosine (8-OHdG) as an effective biomarker on DNA damage from traffic exhaust and/or smoking in exposed and non-exposed individuals. With subject consents, the levels of plasma NOx, urinary 1-hydroxypyrene (1-OHP) and 8- OHdG were determined for 95 male taxi drivers and 75 male community residents as the reference group. After adjusting for associate variables, there was a significant correlation between the levels of urinary 8-OHdG and 1-OHP but not NOx. The average level of urinary 8-OHdG was significantly higher in drivers than in community men (13.4 +/- 4.7 vs. 11 .5 +/- 4.7 mug/g creatinine in mean standard deviation). Compared with non-smoking community men, the multivariate logistic regression showed that the odds ratios (OR) of having elevated levels of urinary 8-OHdG (greater than the overall median value, 12.1 mug/g creatinine) were 6.6 (95% confidence interval (CI) = 2.1-20.8) for smoking community men, 5.0 (95% CI = 1.7-14.7) for non-smoking taxi drivers and 4.6 (95% CI = 1.4-15.0) for smoking taxi drivers. Higher risk was also observed for areca quid chewers compared with non-chewers (OR = 1.6; 95% Cl = 1.1-3.6). In conclusion, taxi driving and smoking may contribute independently to elevated DNA damage using urinary 8-OHdG levels as a sensitive biomarker. This effect is most potent on heavy smokers. (C) 2003 Elsevier Science Ltd. All rights reserved.
Subjects
POLYCYCLIC AROMATIC-HYDROCARBONS
BODY-MASS INDEX
NITROGEN- DIOXIDE
PROFESSIONAL DRIVERS
CIGARETTE-SMOKING
AIR-POLLUTION
Type
journal article