Circadian expression of the permethrin-resistant geneYP9M9 in Aedes aegypti
Date Issued
2009
Date
2009
Author(s)
Liu, Yun
Abstract
The mosquito Aedes aegypti, the major vector of dengue virus in Taiwan, is constantly controlled by insecticides. Under this severe artificial selection pressure, resistant population results in high capability against chemical pesticide. According to the insecticide bioassay, the resistant level of the mosquito against permethrin fluctuated based on the application time of the day. The median knock- down time (KT50) under light condition was significantly longer than that under dark condition in both susceptible and permethrin-resistant strains. This phenomenon implied the expression of the resistant gene underlying the circadian control. The hypothesis would be the expression of permethrin-resistant gene is regulated by clock genes. One of the detoxification genes, cytochrome P450 gene (CYP9M9), expressed in fluctuation within 24 hours which showed high level of expression during day time. To further demonstrate a clock gene, period (per), in controlling the expression of permethrin-resistant gene, RNA interference against per were employed to disrupt the circadian clock. In consequence, the expression of CYP9M9 lost fluctuation and kept at low level whole day. My study provides detail information about circadian control on the expression of a permethrin-resistant gene.
Subjects
Aedes aegypti
permethrin
insecticide-resistance
circadian rhythm
SDGs
Type
thesis
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