Elucidating the Role of 14-3-3β in Gastric Cancer Cells Using Proteomics and Network Analysis
Date Issued
2011
Date
2011
Author(s)
Chuang, Kai-Neng
Abstract
Gastric cancer is the second leading cause of cancer death worldwide. Lacking of early detection marker for this disease is the major reason of such high death rate. In our previous study, we found that 14-3-3β protein levels were evaluated in tumor tissues compared with normal tissues, and serum 14-3-3β levels were also significantly higher than control samples. Higher serum 14-3-3β levels correlated with reduced patient sur-vival rate. On the other hand, over-expression of 14-3-3β enhanced growth, invasiveness and migratory abilities of tumor cells. These results suggest that 14-3-3β is a potential biomarker for detection and prognosis in gastric cancer and regulates aggressive phenotypes of tumor cells. To further elucidate the regulatory mechanism of 14-3-3β on tumor progression, we integrated proteomics and network analyses to construct 14-3-3β-regulated protein-protein interaction networks and their enriched biological functions. Key functional relationships of the networks were revealed, including cell death, apoptosis and phosphorylation. Interestingly, we identified a series of heat shock proteins and other apoptosis related proteins, which had been reported to be involved in malignant progression. These proteins were significantly up-regulated in 14-3-3β-over-expressing tumor cells, and the related protein-protein interaction network is highly connected with cell proliferation and apoptosis, indicating that 14-3-3β could affect growth of tumor cells through regulating these proteins expression. In conclusion, 14-3-3β has a high oncogenic potential in gastric cancer and affects tumor progression through its regulating network.
Subjects
gastric cancer
proteomics
14-3-3bea
SDGs
Type
thesis
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