利用骨髓幹細胞及聚乳酸幫助受損肌腱及韌帶組織之修補
Date Issued
2004
Date
2004
Author(s)
王至弘
DOI
922320B002106
Abstract
Poly (lactic acid) is a biodegradable biomedical material that has been used for
bone and cartilage repair. Biomaterials coated with various ECM have been shown to
promote cell adhesion, proliferation, and differentiation. The purpose of this study is
to develop scaffolds that are suitable with ligament cells attachment and to use
ligament cells derived from bone marrow stem cells to induce tissue regeneration. The
first thing of this project is to examine the cellular behaviors of ligament on various
ECM coated on three kinds of PLLA membranes. Before the proceeding of the
co-culture system of mesenchymal stem cell (MSC) and ligament cells, we develop
some method to differentiate BMSC from ligament cells. First we used the flow
cytometry to determine a lot of surface antigens of mesenchymal stem cell like
CD105, CD29, CD44, CD71, and CD34. The differentiated fibroblasts are examined
by scanning electron microscopy, immunostaining of α- smooth muscle antibody.
Furthermore, we develop a novel electrophoretic analysis for characterization. Since
there are no definite markers for fibroblasts in the literature, our unique study will
provide important information for further characterization of ligament or tendon
fibroblasts.
Publisher
臺北市：國立臺灣大學醫學院骨科
Type
journal article
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