Comparison of two fp25k genes from Perina nuda and Lymantria xylina multiple nucleopolyhedroviruses and their expression in insect cells
Date Issued
2005
Date
2005
Author(s)
Wang, Tai-Chuan
DOI
zh-TW
Abstract
The nucleus of an infected cell by nucleopolyhedrovirus is filled with occlusion bodies (OBs) embeded by polyhedrin in the late infection phase. According to the numbers of polyhedra per cell, two types of cytopathic effect (CPE) can be divided, multiple polyhedra (MP; >10 OBs/cell) and few polyhedra (FP; < 10 OBs/cell). FP phenotype is associated with fp25k gene which encodes 25 kDa. When the fp25k sequence is deleted or inserted by the foreign DNA, it causes reducing numbers of OBs in the infected cells. The fp25k of Perina nuda multiple nucleopolyhedrovirus (PenuMNPV) and Lymantria xylina multiple nucleopolyhedrovirus (LyxyMNPV) contained 624 and 651 base pairs, respectively. The former is closely related to Orgyia pseudotsugata multiple nucleopolyhedrovirus (OpMNPV) and the later is to Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV). Both PenuMNPV and LyxyMNPV fp25k genes are late genes containing a transcriptional initiation site, TAAG, of late and very late gene motifs. FP25K protein possessed with coiled-coil and putative actin-binding domains by computer prediction. A phylogenetic tree was constructed based on fp25k sequences, PenuMNPV and LyxyMNPV belonged to group Ⅰ and group Ⅱ, respectively. FP25K protein was confirmed that it is a structure protein and expressed in late phase by Western blotting hybridization. Both PenuMNPV (Ө52) and LyxyMNPV (Ly2) in our lab strains displayed MP phenotype. LyG (LdMNPV-like virus) from infected Lymantria xylina exhibited FP phenotype in infected cells, and its fp25k was deleted 44 nucleotides comparing with the fp25k of LdMNPV. Ly5 strain exhibited two phenotypes, MP and FP in infected LD cells, its fp25k sequence contained wild and deleted type. Therefore, the expression of fp25k or deleted fp25k directly affected the CPE (MP or FP) phenotype of infected cells.
Subjects
樹透翅毒蛾核多角體病毒
黑角舞蛾核多角病毒
fp25k 基因
Perina nuda multiple nucleopolyhedroviruses
Lymantria xylina multiple nucleopolyhedroviruses
fp25k gene
Type
thesis
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